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Collections of defined cDNA fragments and open reading frame DNA sequences that have been cloned into vectors for the purpose of easy identification and isolation for further study. Ideal for use in cloning experiments.
Recombinant human tag-free Tau-441 was co-expressed with DYRK1A in E. coli cells. The Tau-441 was phosphorylated by DYRK1A in vivo and in vitro prior to the final chromatography purification.
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Recombinant full length human AMPK (combination of A1/B1/G3 subunits) was expressed by baculovirus in Sf9 insect cells using the N-terminal GST and C-terminal His tags.
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Recombinant full length human p110 alpha (N345K) mutant subunit and human p85 alpha wild-type subunit were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
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